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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
| Topic 1: Hematology | 20-25% | - Routine and automated hematology procedures
- Red blood cell physiology and disorders
- Hemostasis and coagulation testing
- White blood cell morphology and diseases
|
| Topic 2: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage
- Antibody detection and identification
- Transfusion reactions and adverse events
- Compatibility testing
- Blood group systems and antigens
|
| Topic 3: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring
- Toxicology and liver/kidney function tests
- Electrolytes, acid-base balance, and blood gases
- Carbohydrates, lipids, proteins, and enzymes
|
| Topic 4: Microbiology | 15-20% | - Specimen collection and culture techniques
- Bacteriology and antimicrobial susceptibility
- Identification of pathogenic microorganisms
- Mycology, parasitology, and virology
|
| Topic 5: Immunology and Serology | 5-10% | - Infectious disease serology
- Immunologic testing methodologies
- Immune system principles and components
- Autoimmune and immunodeficiency disorders
|
| Topic 6: Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation
- Pre-analytical, analytical, and post-analytical processes
- Quality control and quality assurance
- Laboratory safety and regulatory compliance
|
| Topic 7: Urinalysis and Other Body Fluids | 5-10% | - Physical, chemical, and microscopic analysis
- Urine formation and composition
- Semen and amniotic fluid testing
- Cerebrospinal, serous, and synovial fluids
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
1. Isolation and detection of Gardnerella vaginalis from vaginal secretions is improved by which of the following?
A) utilization of Thayer-Martin agar
B) incubation at 35-37ÂșC
C) utilization of human blood agar
D) cold enrichment
2. Blood bank
Once the seal on a unit of packed red cells is broken, how long can the unit be stored refrigerated prior to administration:
A) 12 hours
B) 4 hours
C) 24 hours
D) 48 hours
3. If your reactions are strong at immediate spin (3+) and then get weaker at AHG (w+), it could mean the presence of a strong cold antibody.
Cold antibodies tend to be IgM and their optimum phase for reactivity is immediate spin. Incubation and washing of the sample may cause the agglutination that occurred at room temperature to break down. This would appear as a weaker reaction at AHG.
If the reaction strengths varied in each panel cell then that could be an indication that there are multiple antibodies present.
Your screen cells are 3+ at immediate spin and weak (W)+ at AHG. Your auto control is negative for both phases. Some of your antibody panel cells are 3+ at immediate spin and negative at AHG. What should you suspect?
A) A cold antibody may be present
B) A warm autoantibody is present
C) Bad specimen draw
4. Immunology
The immunoglobulin molecule is made up of both heavy and light chains - the light chains can be comprised of which of the following:
A) Alpha or lambda
B) Alpha or beta
C) Kappa or lambda
D) Kappa or beta
5. An employer who fails to provide sufficient personal protective equipment (PPE) for employees may be fined by the:
A) Food and Drug Administration (FDA)
B) Clinical and Laboratory Standards Institute (CLSI)
C) Occupational Safety and Health Administration (OSHA)
D) Centers for Disease Control and Prevention (CDC)
Solutions:
Question # 1 Answer: C | Question # 2 Answer: C | Question # 3 Answer: A | Question # 4 Answer: C | Question # 5 Answer: C |