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American Society of Microbiology ABMM Sample Questions:

1. A patient develops a severe diarrheal illness after consuming raw seafood. Stool culture yields a Gram- negative, comma-shaped bacterium that is oxidase-positive and grows on thiosulfate-citrate-bile salts-sucrose (TCBS) agar, producing blue-green colonies. The MOST significant virulence factor contributing to the severe, cholera-like diarrhea caused by this organism is:

A) Heat-stable enterotoxin (ST)
B) Heat-labile toxin (LT)
C) Type III secretion system (T3SS)
D) Cholera toxin (CT)-like enterotoxin

2. Which virus is recognized as the leading cause of sporadic, severe gastroenteritis in infants and young children globally, for which effective vaccines are now available?

A) Norovirus
B) Rotavirus
C) Astrovirus
D) Enteric Adenovirus (serotypes 40, 41)

3. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Aspergillus
B) Candida
C) Mucor
D) Rhizopus

4. A clinical microbiology laboratory is using flow cytometry to analyze lymphocyte subsets in a patient with suspected immunodeficiency. Which of the following cell surface markers is typically used to identify helper T cells?

A) CD56
B) CD4
C) CD8
D) CD19

5. A research team is investigating the development of a novel vaccine against influenza virus. They are focusing on conserved epitopes within the hemagglutinin (HA) and neuraminidase (NA) proteins to elicit broadly neutralizing antibodies. Which of the following approaches is MOST likely to achieve this goal?

A) Designing a subunit vaccine that presents highly variable regions of HA and NA.
B) Utilizing a recombinant viral vector to deliver conserved epitopes of HA and NA.
C) Administering high doses of inactivated influenza vaccine annually.
D) Developing a live-attenuated influenza vaccine containing multiple serotypes.

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