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ASCP ASCP-MLT Exam Syllabus Topics:

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. Caffeine benzoate solution is used to split the unconjugated bilirubin protein complex releasing the bilirubin so that it can react with diazotised sulphanilic acid. The tartrate buffer creates an alkaline solution and converts the red acid bilirubin to a green coloured compound which can be measured spectrophotometrically.
Which substance is used in the Jendrassik-Grof method to accelerate the reaction of unconjugated bilirubin with the diazo reagent?

A) acetic acid
B) N-butanol
C) caffeine
D) NADH

2. The laboratory uses flammable chemicals for processing and staining patient specimens. One type of chemical used is ethanol. What is the proper way to store this chemical in the laboratory?

A) In the back of the fume hood
B) Under the sink in the laboratory
C) On the shelf with other hazardous chemicals
D) In a flame-resistant cabinet

3. HbsAg is positive in acute and chronic Hepatitis B infections, since the antigen is found on the actual surface of the virus. HbeAg is present in the blood when the hepatitis B viruses are replicating, indicating an active infection. Anti-Hbc IgM is present due to the immune response to the presence of the hepatitis core antigen and indicates an acute infection. Anti-HBs is generally interpreted as indicating recovery and immunity from hepatitis B virus infection, according to the CDC.
Given the following results, what is the immune status of the patient?
HbsAg: positive
HbeAg: positive
Anti-HBc IgM: positive
Anti-HBs: negative

A) susceptible
B) immunization
C) acute infection
D) chronic infection

4. Amniotic fluid bilirubin is increased in association with the severity of hemolytic diseases of the newborn. As red blood cells lyse during these conditions, bilirubin builds up as a byproduct of the red cell destruction. The more red blood cells that are being destroyed in the baby, the more increased the bilirubin level will become.
Which one of the following tests BEST correlates with the severity of hemolytic disease of the newborn (HDN).

A) Rh antibody titer of baby's blood
B) amniotic fluid bilirubin
C) antibody titer of mother's blood
D) L/S ratio

5. A young man is experiencing difficult breathing after fainting. The physician orders a blood gas analysis which shows the following results:
pH = 7.25
pCO2 = 62 mmHg
pO2 = 70 mmHg
HCO3 = 23 mEq/L
Which condition is most likely afflicting this patient?

A) metabolic alkalosis
B) metabolic acidosis
C) respiratory alkalosis
D) respiratory acidosis

Solutions: